Abstract Detail

Reproductive biology

Thilges, Katherine [1], Chamberlin, Mark [1], Albertsen, Marc [2], Horner, Harry [3].

A combined microscopic study of a male-sterile, female-sterile line in Glycine max.

A completely male-sterile, female-sterile mutant was identified in a transformation study for sterility in soybean (Glycine max L.). This double-sterile mutant exhibited abnormalities in both the male and female organs during micro- and mega-sporogenesis, and gametogenesis. Vegetatively, the mutant line showed no differences from the wild-type line. Analyses using squashes and a fluorescent dye staining of the chromosome behavior during male meiosis was done to better understand any issues that could occur that would cause sterility. Clearings of aldehyde-fixed wild-type and mutant anthers and ovules were done throughout their development, followed by confocal laser scanning microscopy and optical sectioning to compare any abnormalities, and to determine the timing of mutant abortion in both the male and female organs. Additionally, scanning electron microscopy was used to observe the differences between wild-type and mutant anthers and male cells prior to and following mutant abortion. Preliminary results indicate there are chromosomal abnormalities during male mutant meiosis. Mutant megasporogenesis proceeds through meiosis and the early formation of a megagametophyte when development ceases. Mutant anther development proceeds through the released microspore stage after which the resulting male cells become irregular and eventually abort. These first-time results will be compared with an already published molecular study of this double-sterile mutant and will provide insights as to the specific mechanism causing this dual sterility.

1 - DuPont Pioneer, Trait Discovery and Optimization, 7100 NW 62nd Ave, Johnston, IA, 50131, USA
2 - DuPont Pioneer, 7250 NW 62nd Ave. PO Box 552, JOHNSTON, IA, 50131-0552, USA
3 - Iowa State University , Genetics, Development, and Cell Biology & Microscopy and Nanoimaging Facility, 2200 Osborn Drive, Ames, IA, 50011-4009, USA

Keywords:
none specified

Presentation Type: Oral Paper
Session: 42, Reproductive Biology
Location: 205/Savannah International Trade and Convention Center
Date: Wednesday, August 3rd, 2016
Time: 11:00 AM
Number: 42004
Abstract ID:107
Candidate for Awards:None