Abstract Detail

Genomics / Proteomics

Zhang, Heng-You [1], Song, Qijian [2], Luo, Yan [3], Nelson, Randall L. [4], Song, Bao-Hua [5].

Genome-wide association study of soybean cyst nematode (Heterodera glycines Ichinohe) HG type 2.5.7 (race 1) resistance in wild soybean (Glycine soja Sieb. & Zucc.).

Soybean cyst nematode (SCN) is one of the most destructive pathogens of soybean plants worldwide. Thus far, most of the commercial SCN- resistant soybean cultivars have been developed from very limited resistant germplasm resources. Over use of these limited resistant sources has resulted in SCN race shifts in many soybean-growing areas due to evolutionary arm race. To broaden SCN-resistance breeding resources and mitigate nematode damage, a genome-wide association study (GWAS) of SCN race 1 resistance was conducted with an association panel containing 1035 wild soybean accessions using 41,087 single nucleotide polymorphisms (SNPs). A total of 15 SNPs distributed on nine chromosomes were detected to be significantly associated with SCN race1 resistance, and two SNPs mapped in previously identified QTLs, S3-10 on chromosome 11 and q18 on chromosome 18. Importantly, one of the peak SNPs is tightly close to the functionally validated SNP, B1-7. Plant disease defense related genes encoding various types of protein kinases (PK), zinc finger domain containing proteins, pathogenesis-related proteins, and glutathione synthetase, and RING domain proteins were also identified within the linked regions of the peak SNPs. Our findings highlight the potential of using wild soybean germplasms to identify candidate genes associated with SCN resistance. The identified SNPs and candidate genes can benefit breeding and further facilitate dissecting the molecular mechanisms of soybean-SCN interactions. All of these efforts will be focused on ensuring food security by developing resilient soybean varieties in the face of global climate change and population growth.

1 - University of North Carolina at Charlotte, Department of Biological Sciences, Charlotte, NC , 28223, USA
2 - United States Department of Agriculture, Genomics and Improvement Laboratory, Agricultural Research Service, Beltsville, MD , 20705, USA
3 - University of North Carolina at Charlotte, Department of Biological Sciences, Charlotte, NC, 28223, USA
4 - University of Illinois, Department of Crop Sciences, Urbana , IL, 61801, USA
5 - University Of North Carolina At Charlotte, Biology, 9201 University City Blvd, , NC, Charlotte, NC, 28223-0001, USA

Keywords:
Soybean cyst nematode
Genome-wide association study
Glycine soja
single nucleotide polymorphisms
resistance.

Presentation Type: Poster
Session: P, Genomics/Proteomics Posters
Location: Exhibit Hall/Savannah International Trade and Convention Center
Date: Monday, August 1st, 2016
Time: 5:30 PM This poster will be presented at 5:30 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PGP003
Abstract ID:181
Candidate for Awards:None