| Abstract Detail
The Odum Legacy: Plant Systems Across Scales Wang, Jack P. [1], Sederoff, Ronald R. [2], Chiang, Vincent L. [2]. Complete Proteomic Based Enzyme Reaction and Inhibition Kinetics Reveal How Monolignol Biosynthetic Enzyme Families Affect Metabolic-Flux and Lignin. We established a predictive kinetic metabolic-flux model for the 21 enzymes and 24 metabolites of the monolignol biosynthetic pathway using Populus trichocarpa secondary differentiating xylem. To establish this model, a comprehensive study was performed to obtain the reaction and inhibition kinetic parameters of all 21 enzymes based on functional recombinant proteins. 104 Michaelis-Menten kinetic parameters and 103 inhibition kinetic parameters were derived from these enzymes. Through mass spectrometry, we obtained the absolute quantities of all 21 pathway enzymes in the secondary differentiating xylem. These absolute protein quantities and kinetic parameters are the most extensive experimental data generated from a single tissue specialized in wood formation. We used these data to construct the predictive kinetic metabolic-flux model to provide the most comprehensive mathematical description of the monolignol biosynthetic pathway. The model was validated using experimental data from transgenic P. trichocarpa plants. The model predicts how pathway enzymes affect lignin content and composition, explains a long standing paradox regarding the regulation of monolignol subunit ratios in lignin, and reveals novel mechanisms involved in the regulation of lignin biosynthesis. This model provides the best explanation to date of the effects of genetic and transgenic perturbations of the monolignol biosynthetic pathway in flowering plants.
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1 - North Carolina State University, Forestry and Environmental Resources, 840 Main Campus Drive, Partners II Bldg, Raleigh, NC, 27695, USA
2 - North Carolina State University, 840 Main Campus Drive, Partners II Bldg, Raleigh, NC, 27695, United States
Keywords:
Lignin Biosynthesis
Predictive Model
Metabolic Flux
Enzyme Kinetics.
Presentation Type: Symposium Presentation
Session: SY02, The Odum Legacy: Plant Systems Across Scales
Location: Chatham Ballroom - C/Savannah International Trade and Convention Center
Date: Monday, August 1st, 2016
Time: 9:00 AM
Number: SY02002
Abstract ID:282
Candidate for Awards:None |
