Abstract Detail

Molecular Ecology and Evolution

Hoekstra, Paul [1], Carvalho Lopes, Jenifer de [2], Brandão, Rita [3], Mello-Silva, Renato [4], Smets, Erik [1], Chatrou, Lars [5].

Correlated substitution rates across genomic compartments in Annonaceae.

Substitution rates can differ greatly among and within lineages. These different rates of molecular evolution have been linked to a range of causes, such as generation time, DNA repair mechanisms and life history traits. Understanding the patterns and mechanisms of variation in substitution rates is vital for understanding evolution.
In this study we explored the variation in substitution rates within the family Annonaceae. This plant family is confined to the tropics around the world with one exception in the temperate regions of the United States. It consists of four subfamilies, the early diverging Anaxagoreoideae and Ambavioideae and the derived Annonoideae and Malmeoideae. The latter two subfamilies are sister taxa, and each have more than 700 species. Plastid phylogenetic trees have shown markedly different substitution rates between the two clades. In this study we have assembled the chloroplast genomes and nuclear ribosomal DNA using Illumina HiSeq data of 22 Annonaceae, 10 specimens of both Annonoideae and Malmeoideae and one specimen of both Anaxagoreoideae and Ambavioideae. We found that the differences in substitution rates between the two large subfamilies is also present in nuclear rDNA, and show patterns similar to substitution rates in plastid markers. The differences in substitution rates in Annonaceae are thus not caused by biparental inheritance of plasmids neither by failure of one of the chloroplast or nuclear based DNA repair mechanisms. Therefore the results of this study reduce the number of hypotheses about the causes of the differences in substitution rates between the two major Annonaceae subfamilies and give an interesting basis for the direction of follow-up studies.

1 - Naturalis Biodiversity Center, Botany section, Darwinweg 2, Leiden, 2300RA, Netherlands
2 - Universidade de São Paulo, Instituto de Biociências, Departamento de Botânica, Rua do Matão 277, São Paulo, SP, 05508-090, Brazil
3 - Maastricht University, Evolutionary Biology and Botany, Kapoenstraat 2, Maastricht, 6211 KW, Netherlands
4 - Universidade de São Paulo, Instituto de Biociências, Rua do Matão 277, São Paulo, SP, 05508-090, Brazilië
5 - Wageningen University, Droevendaalsesteeg 1, WAGENINGEN, N/A, 6708 PB, Netherlands

Keywords:
Next generation sequencing
Annonaceae
Substitution rates
Molecular evolution.

Presentation Type: Oral Paper
Session: 40, Molecular Ecology and Evolution
Location: 102/Savannah International Trade and Convention Center
Date: Wednesday, August 3rd, 2016
Time: 8:00 AM
Number: 40001
Abstract ID:286
Candidate for Awards:Margaret Menzel Award