Abstract Detail

Phylogenomics

Gernandt, David S. [1], Aguirre, Xitlali [2], Vázquez Lobo, Alejandra [3], Willyard, Ann [4], Moreno, Alejandra [5], Jaramillo Correa, Juan Pablo [6], Piñero, Daniel [7], Liston, Aaron [8].

Multilocus phylogenetics of Pinus subsection Australes.

In the genus Pinus, phylogenetic inference of relationships among closely-related species is hampered by incomplete lineage sorting and hybridization. We studied phylogenetic relationships in Pinus subsection Australes, an ecologically and economically important group of New World pines that includes three western North American species with serotinous cones ("the closed cone pines") and approximately 27 species distributed from eastern North America to Central America and the Caribbean. We used solution hybridization for target enrichment (hyb-seq) of 700 low-copy nuclear genes and a mitochondrial gene sequence in more than 100 individuals representing the subsection (an average of > 3 individuals per species). The method also permitted the recovery of high copy nuclear ribosomal DNA and complete plastomes. A subset of these data was used to characterize nucleotide substitution rates and infer time-calibrated gene genealogies and species trees using coalescent approaches. We re-evaluated previous plastid DNA results that were at odds with traditional classifications in recovering Pinus glabra as the sister group to the closed cone pines. Similarly, we evaluated the hypothesis that all or most species in the southeastern United States are descendants of Mexican and Central American ancestors.

1 - Instituto de Biologí­a, UNAM, Apartado Postal 70-233, Coyoacan, Ciudad de Mexico, CM, 04510, Mexico
2 - Instituto de Biologí­a, Apartado Postal 70-233, Coyoacan, Ciudad de Mexico, CM, 04510, Mexico
3 - Universidad Autónoma del Estado de Morelos, Centro de Investigación en Biodiversidad y Conservación, Av. Universidad No. 1001, Col Chamilpa, Cuernavaca, MOR, 62209, Mexico
4 - Hendrix College, Biology Dept, 1600 Washington Ave, Conway, AR, 72032, USA
5 - Instituto de Biologí­a, UNAM, Jardí­n Botánico, Circuito exterior S/N Ciudad Universitaria, Coyoacán, Mexico, CM, 04510, Mexico
6 - Universidad Nacional Autónoma de México, Instituto de Ecologí­a, Apartado Postal 70-367, CoyoacÁn, Ciudad de Mexico, CM, 04510, Mexico
7 - Universidad Nacional Autónoma de México, Instituto de Ecologí­a, Apartado Postal 70-275, CoyoacÁn, Ciudad de Mexico, CM, 04510, Mexico
8 - Oregon State University, Department Of Botany & Plant Pathology, 2082 Cordley Hall, Corvallis, OR, 97331-2902, USA, 541/737-5301

Keywords:
Coalescence
mtDNA
low-copy nuclear markers
pine
Plastome.

Presentation Type: Oral Paper
Session: 1, Phylogenomics I
Location: 101/Savannah International Trade and Convention Center
Date: Monday, August 1st, 2016
Time: 10:15 AM
Number: 1009
Abstract ID:332
Candidate for Awards:None