Abstract Detail

Systematics

Waight, Emiko [1], Thompson, Coleen [1], Blischak, Paul [2], Tian, Yuan [2], Gaither, Jeff [3], Kubatko, Laura [4], Wolfe, Andrea D. [5].

Extension and application of methods for detecting hybridization in the plant genus Penstemon.

The goal of our research was to test new methods of analysis for detecting hybrids on a known group of hybridizing species of the plant genus Penstemon (Plantaginaceae). Five species (P. centranthifolius, P. spectabilis, P. X parishii, P. clevelandii, and P. grinnellii) were selected for this study because previous experiments have shown that hybridization occurs among them in the wild, which makes them an appropriate group for testing new methods of analysis. These new methods include the Hills test and a modified STRUCTURE analysis. Previous methods of analysis relied on comparing separate estimates of gene trees to look for patterns consistent with hybridization and were often unreliable for analyzing hybrid speciation or detecting introgressive hybridization. By contrast, the Hils Test calculates a test statistic directly from observed DNA sequence data without using gene tree estimates, and is capable of efficiently analyzing large quantities of data. STRUCTURE is not ordinarily used to analyze sequence data so we tried a variety of approaches to code and sample our data for use within the program. The data collected to test these methods consisted of forty-four individuals sequenced at twelve loci for a total of 5,002 base pairs. The tests showed extensive hybridization between the species and supported existing hypothesized hybrid relationships. These new tests can be used in future hybrid studies for quicker, more accurate predictions of hybrid relationships

1 - Ohio State University, Evolution, Ecology, and Organismal Biology, 318 W. 12th Ave, Columbus, OH, 43210, USA
2 - Ohio State University, Evolution, Ecology and Organismal Biology, 318 W. 12th Ave, Columbus, OH, 43210, USA
3 - Ohio State University, Mathematical Biosciences Institute
4 - Ohio State University, Statistics, Columbus, OH, 43210, USA
5 - Ohio State University, Department Of Ecology, Evolution, And Organismal Biology, 318 W. 12th Avenue, COLUMBUS, OH, 43210-1293, USA

Keywords:
methods
phylogenetics
Penstemon
hybridization.

Presentation Type: Poster
Session: P, Systematics Section/ASPT Posters
Location: Exhibit Hall/Savannah International Trade and Convention Center
Date: Monday, August 1st, 2016
Time: 5:30 PM This poster will be presented at 6:15 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PSY042
Abstract ID:425
Candidate for Awards:None