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Abstract Detail



Conservation Biology

Cohen, Jim [1].

Population genetics of Oreocarya crassipes, an endangered species.

Oreocarya crassipes (Boraginaceae) is an endangered species endemic to Brewster Co., Texas in the area just north of Big Bend National Park. O. crassipes is known from a small number of populations along the Fizzle Flat Lentil, and the species has edaphic specialization within the area. While aspects of the ecology of the species are known, the genetic and genomic diversity and population structure have not been examined. Leaf samples from 192 plants from across four populations were collected for genetic and genomic studies. Using tunable genotyping-by-sequencing (tGBS), single nucleotide polymorphisms (SNPs) from across the genome were identified. Datasets with varying numbers of SNPs and missing data were compiled, and genomic diversity and population structure were studied using hierfstat and fastSTRUCTURE, respectively. Results of population structure analyses suggest that the sampled individuals comprise two genetically structured groups, although population structure is also observed should the sampled individuals be divided among three groups. In general, FST values of the populations are approximately 0.05, suggesting little differentiation across the sampled populations, although FST values are greater for some pairs of the four sampled populations. Additionally, FIS values tend to range from slightly greater than 0 to 0.15, suggesting that inbreeding occurs across the sampled populations. As the number of SNPs increases, along with a greater amount of missing data, FST and FIS values increase as well.


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Keywords:
Boraginaceae
population genomics
genetic structure
Oreocarya
Conservation genetics.

Presentation Type: Poster
Session: P, Conservation Biology Posters
Location: Exhibit Hall/Savannah International Trade and Convention Center
Date: Monday, August 1st, 2016
Time: 5:30 PM This poster will be presented at 6:15 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PCB006
Abstract ID:514
Candidate for Awards:None


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