Abstract Detail

Genomics / Proteomics

Evans, Timothy [1], Weitzel, Alexander [2], Faden, Robert [3].

Examination of the molecular genetic basis of andromonoecy in Aneilema (Commelinaceae).

Many species of the plant genus Aneilema (Commelinaceae) exhibit an andromonoecious breeding system: a single plant will produce both hermaphroditic and staminate flowers. The type of flower that will develop at a given time follows a predictable sequence in the first inflorescences produced by a plant, with flowers formed during the first 7-9 days being hermaphroditic and those formed later being staminate. As a result, Aneilema provides an opportunity to examine the molecular mechanisms underlying the production of each floral type. To identify candidate genes that may be involved in determination of floral type, we generated de novo transcriptome sequences from young floral buds of Aneilema schlechteri plants grown from seed, including day-two (hermaphroditic) and day-fifteen (staminate) buds, as well as a floral bud from Tradescantia ohiensis, a member of Commelinaceae that produces only hermaphroditic flowers. A de novo reference assembly consisting of the three types of floral buds resulted in 196,428 transcripts. Differential expression analyses were conducted to determine candidate genes for determination of floral type. Efforts are currently underway to annotate and categorize each transcriptome and the differentially expressed genes. This study provides a foundation upon which the molecular genetics of floral diversification in Aneilema and Commelinaceae as a whole will be examined.

1 - Grand Valley State University, Biology, 3321 Kindschi Hall, 1 Campus Drive, Allendale, MI, 49401, United States
2 - Grand Valley State University, Biology, 1 Campus Drive, Allendale, MI, 49401, United States
3 - Smithsonian Institution, Botany, MRC-166 National Museum of Natural History, PO Box 37012, Washington, DC, 20013-7012, USA

Keywords:
Aneilema
Commelinaceae
Transcriptome
differential expression.

Presentation Type: Poster
Session: P, Genomics/Proteomics Posters
Location: Exhibit Hall/Savannah International Trade and Convention Center
Date: Monday, August 1st, 2016
Time: 5:30 PM This poster will be presented at 5:30 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PGP007
Abstract ID:635
Candidate for Awards:None