Abstract Detail

Phylogenomics

Jabaily, Rachel Schmidt [1], Gardner, Andy [2], Sessa, Emily Butler [3], Michener, Pryce [4], Johnson, Eden [5], Shepherd, Kelly A. [6].

Molecules, morphology, and monophyly: revising the enigmatic Australian clade Goodenia s.l. (Goodeniaceae) using comprehensive sampling and big data.

Previous phylogenetic efforts by our research team have identified the clade Goodenia s.l. as the most species rich and taxonomically challenging in the predominantly Australian plant family Goodeniaceae. The largest genus, Goodenia, is not supported by consistent morphological characters. Molecular phylogenetics resolve it into three major clades with multiple embedded smaller genera, which are individually variously supported by both molecular and morphological data. We have sought to identify and describe monophyletic clades as genera within Goodenia s.l. Our ultimate efforts at fully exploring the phylogeny of Goodenia s.l. have included nearly complete sampling of 220+ described species, as well as many undescribed, potentially new species. Sequences of loci trnL-trnF, matK, and nrITS for all included species were scaffolded onto a strongly supported phylogenetic backbone from next generation genome skimming sequencing of 28 target species. Draft plastome analysis found maximum phylogenetic support for all clades except several key nodes within Goodenia Clade C, the most morphologically variable and taxonomically complex clade of Goodenia s.l. Mitochondrial exomes yield very high phylogenetic support, and potential phylogenetic conflict between chloroplast, mitochondrial, and nuclear histories was fully explored particularly within Clade C. New genera to be described within Goodenia s.l. encompass considerable morphological variability, particularly with convergence in floral form, but now will have strong support from both morphological and molecular data.

1 - Rhodes College, Botany, 2000 N. Parkway, Memphis, TN, 38112, USA
2 - California State University Stanislaus, Department of Biological Sciences, One University Circle, Turlock, CA, 95382, USA
3 - University of Florida, Biology, PO Box 118525, Gainesville, FL, 32611, USA
4 - Rhodes College, Biology, 2000 N. Parkway, Memphis, TN, 38112, USA
5 - University of Missouri, Department of Biological Sciences, 105 Tucker Hall, Columbia, MO , 65211
6 - Western Australian Herbarium, 17 Dick Perry Avenue, Technology Park, Western Precinct, Kensington, WA, 6151, Australia

Keywords:
taxonomy
revision
Australia
Asterales
Goodeniaceae
genome skimming
chloroplast
Mitochondria.

Presentation Type: Oral Paper
Session: 16, Phylogenomics II
Location: 202/Savannah International Trade and Convention Center
Date: Tuesday, August 2nd, 2016
Time: 11:15 AM
Number: 16013
Abstract ID:636
Candidate for Awards:None