Abstract Detail

Conservation Biology

Nnamani, Catherine V [1], Igwe, David O [2], Ogbogo, Ikechukwu. B [3].

Characterization of genetic variability in Azadrachta indica A. Juss Accessions from South-east Nigeria using Random Amplified Polymorphic DNA (RAPD) Markers.

Abstract Azadirachta indica A. Juss, is one of two species in the genus Azadirachta. It is a multipurpose plant of high commercial importance due to its medicinal properties and plethora of biological activities of the bioactive components contained in its seeds, leaves and bark against a wide range of pests and pathogens.. Knowledge of relative genetic distance among genotypes is useful in a drug discovery and it permits organization of germplasm for resources conservation. Ten accessions of Azadirachta indica from Ebonyi State, Nigeria were extracted using CTAB method and amplified using ten RAPD markers. Phylogenetic reconstruction was done using UPGMA option in NTSYSpc (version 2.2). Out of the ten markers, five were reproducibly found to be polymorphic. A total of 65 allelic variants were obtained, yielding 90.4% polymorphism. The accessions were clustered into three major clades with bootstrap values ranging from 64 to 89%, with the highest and lowest values in clades I and II, respectively. The dissimilarity maximum value was 1 while that of the minimum was 0.283 at 95% percentile value of 0.667. The ten RAPD markers had the range of alleles from 10-15 with a mean of 4.93. Polymorphic information content (PIC) values were between 0.365 and 0.768 with an average of 0.645. Similarity indices ranged from 0.60 to 0.94. The markers such as OPT-11 and OPH-1, OPT-1, OPT-2 and OPT-7 were found to be more discriminatory and polymorphic than others. Also, the Azadirachta indica populations from diverse agro-climatic regions in the State clustered into three major coordinates, revealing a wide genetic diversity among the characterized accessions. Thus, the use of RAPD markers could be useful molecular tools to characterize the diversity of Azadirachta indica to complement the role of morphotaxonomy. This could also help in proper identification by the pharmaceutical industries for drug discovery

1 - Ebonyi State University Abakaliki, Nigeria, Applied Biology, Ebonyi State University Abakaliki, Nigeria, Abakaliki, Ebonyi, PMB053
2 - International Institute of Tropical Agriculture (IITA), Nigeria, Virology and Molecular Diagnostics Laboratory, Ibadan, Oyo, +234
3 - Nnamani, Catherine V, Applied Biology, Abakaliki, Ebonyi

Keywords:
Azadirachta indica.

Presentation Type: Poster
Session: P, Conservation Biology Posters
Location: Exhibit Hall/Savannah International Trade and Convention Center
Date: Monday, August 1st, 2016
Time: 5:30 PM This poster will be presented at 5:30 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PCB001
Abstract ID:66
Candidate for Awards:None