Abstract Detail

Bryology and Lichenology

Rahmatpour, Nasim [1], Goffinet, Bernard [1].

The genome of Funaria hygrometrica, insights of transcriptome on structure and evolution.

The Funariaceae hold several species that have served as model taxa for developing our understanding of moss developmental and reproductive biology. Genomic resources are well established for Physcomitrella patens enhancing comparative genomics in land plants and also among moss taxa. However, the genomic resources for mosses remain scarce, and hence the developmental genetic networks remain known poorly. Here we are characterizing the transcriptome of the gametophyte of Funaria hygrometrica, a species related to Physcomitrella patens. Total RNA was extracted from 100mg tissue of gametophyte tissue including rhizoids, stem and leaves from three replicates, purified mRNAs were sequenced by NextSeq 500 Mid output afterwards the de-novo assembly of high quality reads was done by Trinity and the contigs of each replicate were clustered together, yielding 47,341 unique transcripts. We blasted contigs to NCBI non-redundant protein database with three maximum number of blast hit and also they were blasted against Swiss-Prot database to have a better functional annotation and Gene Ontology (GO). Here we characterize the diversity of gene families in Funaria hygrometrica, infer evidence of whole genome duplication and contrast this to those of Physcomitrella patens.

1 - University of Connecticut, Ecology and Evolutionary Biology, 75 North Eagleville Road, Storrs, CT, 06269, United States

Keywords:
Transcriptome
Funaria
Physcomitrella
moss.

Presentation Type: Oral Paper
Session: 32, Bryology and Lichenology (ABLS) II
Location: 103/Savannah International Trade and Convention Center
Date: Tuesday, August 2nd, 2016
Time: 2:45 PM
Number: 32004
Abstract ID:668
Candidate for Awards:A. J. Sharp Award