Abstract Detail

Evolutionary Developmental Biology (Evo-Devo)

Han, Jiahong [1], Berger, Brent [2], Ricigliano, Vincent [3], Gardner, Andy [4], Shepherd, Kelly A. [5], Jabaily, Rachel Schmidt [6], Howarth, Dianella [7].

Phylogenetics and expression of CYCLOIDEA-like genes in Goodeniaceae.

Floral symmetry is of special interest in understanding angiosperm evolution and ecology. Shifts in floral symmetry have been common between radial symmetry and bilateral symmetry within angiosperms. Growing evidence from transcription factors in the TCP gene family, CYC-like genes, indicate that dorsal restriction of expression in the corolla and gene duplication are correlated with a shift to bilateral symmetry. In this study, we examined the duplication events around the diversification of Goodeniaceae, a predominately Australian and Pacific Island plant group, which contains both radially and bilaterally symmetrical flowered species. We found the three core eudicot specific clades of CYC-like genes (CYC1, CYC2, and CYC3) in Goodeniaceae. Also we found additional duplications within two clades (CYC2 and CYC3) clades in Goodeniaceae. Additionally, there are additional copies of CYC3 (within CYC3A and CYC3B) in most species of Goodeniaceae with bilaterally symmetrical flowers. Using realtime qPCR, we showed that most of these copies are expressed across floral tissue in the Goodenicaceae species Scaevola aemula. One copy, SaeCYC2 was expressed in a dorsal-ventral pattern as has been found in CYC2 paralogs in other groups. SaeCYC2 was expressed much higher in dorsal petals than in the ventral petals. Conversely, a SaeCYC3 paralog has the highest expression in ventral petals in S. aemula. Taken together, these results indicate that SaeCYC2 may be expressed in a similar pattern to that is other groups, but that SaeCYC3 may have the opposite expression pattern, being more highly expressed ventrally. Based on our phylogeny and expression analyses, we suggest that gene duplication and asymmetry expression have played a major role in diversification of the Goodeniaceae CYC gene family.

1 - St. John's University, Biological Sciences, 8000 Utopia Parkway, Jamaica, NY, 11439, USA
2 - St. John's University, Biological Sciences, 8000 Utopia Parkway, Queens, NY, 11439, USA
3 - USDA-ARS, 2000 E Allen Rd, Tucson, AZ, 85719, USA
4 - CSU Stanislaus, Biological Sciences, One University Circle, Turlock, CA, 95382, USA
5 - Western Australian Herbarium, Department of Parks and Wildlife, Locked Bag 104, Bentley Delivery Centre, Perth, Western Australia, 6983, Australia
6 - Rhodes College, Botany, 2000 N. Parkway, Memphis, TN, 38112, USA
7 - St. John's University, Biological Sciences, St. Albert Hall, 8000 Utopia Pkwy, Jamaica, NY, 11439, USA

Keywords:
floral symmetry
CYCLOIDEA
Goodeniaceae
qPCR
gene expression.

Presentation Type: Poster
Session: P, Evolutionary Developmental Biology (Evo-Devo) Special Topic:Posters
Location: Exhibit Hall/Savannah International Trade and Convention Center
Date: Monday, August 1st, 2016
Time: 5:30 PM This poster will be presented at 5:30 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PEV007
Abstract ID:746
Candidate for Awards:Katherine Esau Award