| Abstract Detail
Bryology and Lichenology Flowers, Nicholas [1], Merced, Amelia [2], Renzaglia, Karen [1]. Callose presence in the spores of Phaeoceros carolinianus. Cell wall polysaccharides are critical to the life and survival of plants on land. Therefore, it is important to identify cell wall components, especially as they relate to development. Callose, a β-1-3 glucan, is a transient polymer that is utilized extensively in many embryophytes during important biological processes. Although callose is known to be involved in spore development and structure in liverworts and mosses, there are contradictory reports on callose in Anthocerotophyta, with the most recent studies reporting an absence of this polysaccharide during sporogenesis in the group. By corroborating data from fluorescent microscopy via aniline blue and transmission electron microscopy via immunogold labeling with anticallose antibodies, we show that callose is indeed present during sporogenesis in Phaeoceros carolinianus. These data provide evidence of the novel internalization of callose associated with the intine and aperture of spores in P. carolinianus. Because it is a hydrophilic molecule, we suggest that callose may be involved in the imbibition and germination of spores in hornworts.
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1 - SIU, Department of Plant Biology, Life Science II, Mail Code 6509, Carbondale, IL , 62901-6509, USA
2 - University of Puerto Rico, Institute of Neurobiology, San Juan, Puerto Rico, 00921, USA
Keywords:
Callose
Anthocerotophyta
Spores
Immunolabeling
Phaeoceros.
Presentation Type: Oral Paper
Session: 32, Bryology and Lichenology (ABLS) II
Location: 103/Savannah International Trade and Convention Center
Date: Tuesday, August 2nd, 2016
Time: 2:15 PM
Number: 32002
Abstract ID:802
Candidate for Awards:A. J. Sharp Award |
