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Abstract Detail

Population Genetics

Jinga, Percy [1], Palagi, Jason [2], Ashley, Mary V. [3].

Development of microsatellite loci of pod mahogany, Afzelia quanzensis (Fabaceae), by Illumina shotgun sequencing, and cross-amplification in Afzelia africana.

Premise of the Study: Microsatellite loci were developed for Afzelia quanzensis as a first step towards investigating genetic diversity and population structure of the species in its native range.
Methods and Results: Illumina shotgun sequencing was used to generate raw sequence reads, which were searched for potential microsatellite loci. A total of 70 potential microsatellite loci were tested for amplification and polymorphism, and 39 successfully amplified. Of the 39 loci that amplified, 12 were polymorphic while 27 were monomorphic. The 12 polymorphic loci were cross-amplified in Afzelia africana, and eight successfully amplified.
Conclusions: The 12 polymorphic microsatellite loci can be used for genetic studies of A. quanzensis, which can help determine its conservation status. Eight loci can also be used for genotyping in A. africana.

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1 - University of Illinois at Chicago, Biological Sciences Department, 845 W. Taylor Street, Rm 1028 SEL West, Chicago, IL, 60607, USA
2 - University of Illinois at Chicago, Biological Sciences Department, 845 W. Taylor Street, Rm 1031 SEL West, Chicago, IL, 60607, USA
3 - University Of Illinois At Chicago, 845 W. Taylor St., Chicago, IL, 60607, USA

none specified

Presentation Type: Poster
Session: P, Population Genetics Posters
Location: Exhibit Hall/Savannah International Trade and Convention Center
Date: Monday, August 1st, 2016
Time: 5:30 PM This poster will be presented at 5:30 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PPG001
Abstract ID:113
Candidate for Awards:Genetics Section Poster Award

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