| Abstract Detail
Recent Topics Posters Tabner, Sara [1], Schnabel, Andrew [1]. Phylogeny of Gleditsia (Leguminosae) and its Relationship to the “Umtiza Clade” Based on Low-Copy Nuclear Genes. Gleditsia (Leguminosae) is a small genus (12â€13 species) of thorny trees with a distribution largely in eastern North America and eastern Asia, but with outlying species in South America and the southern Caucasus. Previous phylogenetic work on the Caesalpinioideae placed Gleditsia in a clade with Gymnocladus (from eastern North America and eastern Asia) and Umtiza (from South Africa). That trio of genera has been hypothesized to be part of a larger Umtiza clade or grade that includes Acrocarpus (India & SE Asia), Arcoa (Caribbean), Ceratonia (NE Africa & Mediterranean region), and Tetrapterocarpon (Madagascar). Gleditsia itself has been suggested to contain three or four subclades. To resolve some of the outstanding questions about relationships between closely related species within those subclades, as well as to investigate the relationship of Gleditsia to other genera in the Umtiza clade/grade, we tested six low-copy nuclear genes with hypothesized phylogenetic utility in the legumes (AGT1, AIGP, AT103, EIF3E, tRALs, and SHMT). We discarded AGT1, EIF3E, and SHMT due to amplification of multiple fragment sizes that could indicate gene duplication or nonâ€specific priming. Initial comparisons of sequence diversity for the remaining three genes indicated that AIGP and tRALs would be the most useful for our study. Phylogenetic analyses using AIGP failed to resolve relationships within Gleditsia. The tRALs data recovered the same four Gleditsia subclades as in previous studies, but with somewhat different relationships among them, and showed no resolution between the two North American Gleditsia species or among several south Asian Gleditsia species. The tRALs data also reaffirmed the close relationship of Gleditsia to Umtiza and Gymnocladus and supported the hypothesis of an Umtiza grade, rather than an Umtiza clade, within the Caesalpinieae grade of legumes.
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1 - Indiana University South Bend, Biological Sciences, 1700 Mishawaka Ave, South Bend, IN, 46615, USA
Keywords:
Gleditsia
Leguminosae
Umtiza
low-copy nuclear markers
Gymnocladus.
Presentation Type: Recent Topics Poster
Session: P, Recent Topics Posters
Location: Exhibit Hall/Savannah International Trade and Convention Center
Date: Monday, August 1st, 2016
Time: 5:30 PM
Number: PRT023
Abstract ID:1216
Candidate for Awards:None |
