Abstract Detail

Molecular Ecology and Evolution

Sheidaei, Masoud [1], Koohdar, Fahimeh [2], Nourmohammadi, Zahra [3].

Population genetics, molecular phylogeny and bogeography of the genus Lallemantia (Lamiaceae).

The genus Lallemantia Fisch. & C.A.Mey. (family Lamiaceae) is a small genus with 5 species. They are herbaceous annual or biennial plants. There has been no thorough molecular phylogenetic study in this genus and no the species relationship and biogeography data are totally lacking. We studied population genetics, molecular phylogeny and species relationship of all five species in the genus Lallemantia, by multilocus ISSR markers and inter-genic spacer of chloroplast genome rpl16 and compared them with morphometric results. We also tried to reveal the probable time of species divergence and identify the probable ancestral distribution area of these species. Population genetic studies of these species revealed significant molecular difference among the studied population by AMOVA, and STRUCTURE analysis identified the probable gene pools in each species studied. Genetic distance was positively correlated with geographical distance but some degree of gene flow occurred among populations. The species were efficiently delimited by morphological characters, as well as ISSR and Cp-DNA markers. PCoA plot of species based on morphological characters, NJ tree and STRUCTURE plot of ISSR data, as well as the ML tree of Cp-DNA, revealed closer affinity between L. baldschuanica , L. conecens, and L. iberica. L. royleana and L. peltata were placed with some distance from these species. BEAST analysis revealed that the oldest node of Lallemantia appeared in Iran about 37 Mya, followed by the node that led to the formation of L. royleana and L. peltata in about 25 Mya. The tree obtained, dates back L. baldschuanica appearance to about 4 Mya. However, active radiation occurred from 1- 2 Mya. The S-DIVA and MCMC approach of RASP produced similar results and suggested three possible ancestral ranges, A (South Khorasan), D (Alborz) and B (Qazvin) for Lallemantia species in Iran. S-DIVA postulates that the ancestors of Lallemantia species originated in North-East region of the country.

1 - shahid beheshti university, biology, velnjak, tehran, tehran, tehran, _________, IRAN
2 - shahid beheshti university, biology, velnjak, tehran, tehran, tehran, -------, IRAN
3 - Science and Research Branch, Islamic Azad University, Biology, tehran, tehran, tehran, -------, IRAN

Keywords:
Genetic diversity
Biogeography
Cp-DNA
ISSR
Lallemantia
phylogeny.

Presentation Type: Oral Paper
Session: 40, Molecular Ecology and Evolution
Location: 102/Savannah International Trade and Convention Center
Date: Wednesday, August 3rd, 2016
Time: 8:30 AM
Number: 40003
Abstract ID:129
Candidate for Awards:None