| Abstract Detail
Genomics / Proteomics Ramsey, Adam Joseph [1], Mandel, Jennifer R [1]. Patterns of cyto-nuclear disequilibrium and the influence of heteroplasmy in wild carrot, Daucus carota (Apiaceae). Cyto-nuclear linkage disequilibrium (LD) is the non-random association of alleles in the nuclear and cytoplasmic organellar genomes. Patterns of cyto-nuclear LD can indicate admixture of two species or divergent populations, positive or negative cyto-nuclear selection, inheritance of organelles in a non-traditional fashion, or heteroplasmy, the occurrence of more than one mitochondrial or plastid genome within an individual. Cyto-nuclear interactions have been shown to play a role in cytoplasmic male sterility (CMS) which occurs in gynodioecious plant species. These species are also implicated in instances of paternal inheritance of organelles. We used the gynodioecious species, Daucus carota ssp. carota, or wild carrot, to investigate cyto-nuclear LD. We genotyped a total of 227 individuals from two North American regions, 92 from the Olympic Peninsula, Washington State, and 135 from Nantucket Island, Massachusetts. We used 15 nuclear microsatellites, two mitochondrial SNPs and one plastid polymorphism to calculate nuclear-nuclear LD, cyto-nuclear LD and LD between organelles both among regions and within regions. We were further able to identify over 100 individuals heteroplasmic for at least one of the organellar loci. Thus we were able to calculate the same sets of LD values for heteroplasmic and homoplasmic (non-heteroplasmic) individuals. Differences were found between several of the LD calculations. Further goals of this project are to include crop carrots (ssp. sativus) to determine if gene flow between wild and crop carrot may play a role in the patterns of LD investigated here.
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1 - University of Memphis, Department of Biological Sciences, Memphis, TN, 38152, USA
Keywords:
Genetic diversity
Genomes
Mitochondria
SNPs
SSRs
Paternal Leakage
plastid.
Presentation Type: Oral Paper
Session: 19, Genomics & Proteomics I
Location: 105/Savannah International Trade and Convention Center
Date: Tuesday, August 2nd, 2016
Time: 10:45 AM
Number: 19011
Abstract ID:230
Candidate for Awards:Margaret Menzel Award |
