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Abstract Detail


Dobbins, Catherine [3], Gernandt, David S. [1], Aguirre, Xitlali [1], Lobo, Alejandra [2], Willyard, Ann [3].

Determining recent hybridization of pinyon pines in a zone of sympatry in northern Arizona.

We investigated whether pinyon pines with a mosaic of morphological traits on the Mogollon Rim in northern Arizona are recent hybrids or a different species. This is complicated by uncertainty regarding one of the putative parents. Recent studies have demonstrated that the single-leaf pinyon in Arizona (currently treated as Pinus monophylla ssp. fallax) is more closely related to P. edulis than it is to P. monophylla (the single-leaf pinyons of Nevada and California). In Arizona, we sampled multiple individual that represent P. monophylla ssp. fallax, multiple individuals that represent P. edulis, and putative hybrids of these two species. We compared these to multiple individuals of P. monophylla from California. We used biotinylated probes to enrich for 700 low-copy nuclear loci prior to Illumina sequencing with 100 bp paired end reads. We also recovered plastome, high-copy nuclear ribosomal, and mitochondrial sequences. We used de novo and reference-guided assembly to arrange the raw reads, and filtered out putative paralogs using multi-sequence alignments. The most reliably aligned genes were used to create gene trees, which were in turn used to build a species tree. As a final step, we used a parametric approach to explore the power of these data to distinguish hybridization from incomplete lineage sorting.

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1 - Instituto De Biologia, UNAM, Apartado Postal 70-233, Coyoacan, Mexico, N/A, 04510, Mexico
2 - Universidad Autonoma del Estado de Morelos, Centro de Investigacion en Biodiversidad y Conservacion, Universidad No. 1001, Cuernavaca, Morelos, 62209, Mexico
3 - Hendrix College, Biology Dept, 1600 Washington Ave, Conway, AR, 72032, USA

low-copy nuclear markers

Presentation Type: Poster
Session: P, Systematics Section/ASPT Posters
Location: Exhibit Hall/Savannah International Trade and Convention Center
Date: Monday, August 1st, 2016
Time: 5:30 PM This poster will be presented at 5:30 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PSY011
Abstract ID:301
Candidate for Awards:None

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