| Abstract Detail
Conference Wide Straub, Shannon C.K. [1], Fishbein, Mark [2], Liston, Aaron [3]. Introduction to Next Generation Sequencing. Next generation sequencing (NGS) describes technologies that produce billions of base pairs of sequence data per experiment. The low cost of these technologies (currently ca. 60 Mbp / dollar) allows an increasing number of biologists to incorporate genome-scale sequencing into their research. The goal of this workshop is to introduce participants to the essentials of NGS, so that they can develop and initiate their own projects in phylogenetics, population genetics, and gene expression using this technology. In the morning, we will describe the available technologies, applications, and protocols for sample preparation and sequencing. We will also describe options for computational infrastructure and analytical pipelines. In the afternoon, participants will then have an opportunity to work with data from the Illumina platform. Topics covered during the hands-on portion will include plastome sequence assembly and probe design for nuclear gene target enrichment (Hyb-Seq). A laptop computer is required. Fees will cover internet access, extension cords for laptops, a coffee break, and lunch.
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1 - Hobart and William Smith Colleges, Department of Biology, 300 Pulteney Street, Geneva, NY, 14456, USA
2 - Oklahoma State University, Dept Of Botany, 301 Physical Science, Stillwater, OK, 74078, USA, 405-564-4652
3 - Oregon State University, Department Of Botany & Plant Pathology, 2082 Cordley Hall, Corvallis, OR, 97331-2902, USA, 541/737-5301
Keywords:
none specified
Presentation Type: Workshop Proposal
Session: WS01, Introduction to Next Generation Sequencing
Location: 204/Savannah International Trade and Convention Center
Date: Sunday, July 31st, 2016
Time: 8:00 AM
Number: WS01001
Abstract ID:31
Candidate for Awards:None |
