| Abstract Detail
Seed-free Plants at the Genomic Scale Medina, Rafael [1], Johnson, Matthew [2], Liu, Yang [3], Budke, Jessica [4], Wilding, Nicholas [5], Hedderson, Terry [6], Wickett, Norm [7], Goffinet, Bernard [8]. Zooming in on the rapid radiation of the Funariaceae. Some of the difficulties of molecular phylogenetics are the reconstructions of evolutionary radiations in which the hierarchy of the tree cannot be resolved. This is often detected as a rapid succession of cladogenetic events with short branch lengths and low supports due to a reduced number of informative sites. This scenario characterizes the crown group of the family Funariaceae, a diverse lineage of fast growing mosses, which includes the model species Physcomitrella patens and Funaria hygrometrica. The rapid radiation in this family relates to a series of reductions of the sporophyte that can provide insights about the mechanisms and frequency of deep morphological transformations. Previous attempts using up to 10 loci were insufficient to resolve the topology of the family tree. In order to multiply the number of informative sites that may untangle this radiation, we opted for a phylogenomic approach, which combines high-throughput sequencing with targeted enrichment. We cultured 91 different accessions of Funariaceae samples (and outgroups), DNA barcoded them to test the identification against the original voucher, and prepared genomic libraries. The libraries were normalized, pooled and enriched using two sets of oligonucleotide baits: one from the exons of 123 organellar genes and a second one with a selection of 800 nuclear genes. The fragments of the libraries that hybridized with the baits were recovered, amplified, and sequenced. We subsequently recovered the reads in silico with HybPiper, aligned, and analyzed them. Inferences from these genomic data confirmed that a) much of the diversity of the Funariaceae arose over a relatively short period of time and b) that the Funarioideae and Funaria are monophyletic whereas Entosthodon composes a paraphyletic group subtending the Physcomitrium complex. A resolved phylogeny opens the door to a critical reconstruction of the evolution of sporophyte architecture of this lineage. Log in to add this item to your schedule
1 - University Of Connecticut, Ecology And Evolutionary Biology, 75 North Eagleville Road, Storrs, CT, 06269-3043, USA 2 - Chicago Botanic Garden, Plant Sciences, 1000 Lake Cook Road, Glencoe, IL, 60626, United States 3 - University of Connecticut, 75 N Eagleville Rd, Storrs, CT, 06269, United States 4 - University Of California - Davis, Department Of Plant Biology, One Shields Avenue, Davis, CA, 95616, USA 5 - University of Cape Town, Department of Biological Sciences, Privatebag, Rondebosch, 7700, South Africa 6 - University of Cape Town, Privatebag, Rondebosch, 7700, South Africa 7 - Chicago Botanic Garden, 1000 Lake Cook Road, Glencoe, IL, 60022, United States 8 - University Of Connecticut, Department Of Ecology & Evolutionary Biology, 75 N. Eagleville Road, U-3043, STORRS, CT, 06269-3043, USA
Keywords: phylogenomics Bryophytes phylogeny Mosses NGS.
Presentation Type: Colloquium Presentations Session: C4, Seed Free Plants at the Genomic Scale Location: Chatham Ballroom - C/Savannah International Trade and Convention Center Date: Wednesday, August 3rd, 2016 Time: 3:00 PM Number: C4007 Abstract ID:317 Candidate for Awards:None |