Abstract Detail

Genomics / Proteomics

Ravirajan, Mangaiyarkarasi [1], Natesanpayer, Nadimuthu [2].

Lectins (haemagglutinins) from the Red Alga Acanthophora spicifera (vahl)Boergesen: Isolation, partial purification and characterization.

Haemagglutinin (lectins) from various sources have been isolated, characterized and experimented in various preparative, analytical studies and biochemistry related areas. Haemagglutinins are the compounds that are agglutinating the blood cells. As the interest of application is growing rapidly, search of new sources for novel compounds as they are living in specialized habitats. But, not much work has been done in these largely available renewable natural resources, the marine macro algae for haemagglutinin. The Red seaweed, Acanthophora spicifera (vahl) Boergesan was collected from the Mandapam area, Rameswaram, Gulf Of Mannar Biosphere Reserve, India. The seaweed extract showed haemagglutinin activity in human blood (A, B, AB and O), chick blood and sheep blood. The haemagglutinin activity of the crude (saline) extract was determined as 1:8, 1:8, 1:4 and 1:4 in human blood cells A, B, AB and O and 1:8 and 1:8 in chick and sheep blood cells, respectively. The crude extract was purified by ammonium sulphate precipitation and Sephadex G-50 column chromatography. The purified lectin increased the activity to 1:4, 1:4, 1:6 and 1:8 in human blood cells A, B, AB and O and 1:8 and 1:16 in chick and sheep blood cells, respectively. Chemical analysis revealed 31.4% of protein, 5.64% of carbohydrate and 13.06% of total amino acids in purified lectin. The IR spectrum analysis of ASL exhibited the functional groups, NH group, amide I and II and Coo-group. HPLC analysis revealed the presence of acidic, hydroxyl and basic amino acids. The purified ASL appeared on SDS-PAGE as a single band and this revealed the homogeneity of the compound. The molecular mass showed 14740D. The lectin was functional in the PH range from 7 to11.

1 - Sri Balaji Vidyapeeth University, Central Inter Disciplinary Research Facility, MGMCRI campus, Puducherry, Puducherry, 607403, India
2 - Awaiyar Government women's college, Botany, Karaikkal, Puducherry, Puducherry, India

Keywords:
Seaweeds
Acanthophora spicifera
Haemagglutinin
Lectin
Column Chromatography.

Presentation Type: Oral Paper
Session: 27, Genomics & Proteomics II
Location: 105/Savannah International Trade and Convention Center
Date: Tuesday, August 2nd, 2016
Time: 4:45 PM
Number: 27013
Abstract ID:334
Candidate for Awards:None