Abstract Detail

Phylogenomics

Mincey, Katherine [1], Melton, Anthony [2], Hall, Nathan [3], Goertzen, Leslie [3], Boyd, Robert Steven [4].

Intraspecific plastid genome variation and phylogenetic structure within the nickel hyperaccumulator Streptanthus polygaloides (Brassicaceae).

Streptanthus polygaloides (Brassicaceae) is an annual nickel hyperaccumulator endemic to the serpentine soils along the western side of the Californian Sierra Nevada. These serpentine areas are often open, steep habitats that are fragmented along the landscape and may be isolated from the nearest patch by many kilometers. Four morphologically and ecologically diverse morphs of S. polygaloides have been described that vary in sepal color, leaf morphology, height as well as geographic location. The morphs range from being widespread along northern and central California to being geographically isolated from all other morphs by approximately one hundred kilometers. To examine whether the four morphs are genetically distinct we examined complete plastid genome sequences assembled from high-throughput sequencing data. Genomic DNA samples from eight populations of S. polygaloides (2 of each morph: yellow, purple, yellow/purple, and undulate) were sequenced on an Illumina HiSeq platform. Plastid genome sequences were assembled in an iterative fashion with contigs confirmed by mapping original reads back to intermediate stages of assembly. All eight full-length plastid genome sequences were annotated and aligned with various outgroup Brassicaceae. Maximum likelihood and Bayesian analyses were performed with multiple partitioning strategies. Phylogenetic analyses strongly support clades containing purple and undulate samples, although there is a strong geographic signal in the phylogeny overall. Numerous morph-specific plastid SNPs were identified that can be tested more widely across the S. polygaloides range with additional sampling. Ongoing research also includes a transcriptome sequence experiment including all of the morphs.

1 - Auburn University, 1309 Gatewood Dr #1102, Auburn, AL - Alabama, 36830, United States
2 - University of Florida, Department of Biology
3 - Auburn University, Department of Biological Sciences
4 - Auburn University, Department Of Biological Sciences, 101 ROUSE LIFE SCIENCES BLDG, AUBURN UNIVERSITY, AL, 36849-5407, USA

Keywords:
hyperaccumulation
Serpentine
chloroplast
phylogenetics
Streptanthus polygaloides.

Presentation Type: Poster
Session: P, Phylogenomics Posters
Location: Exhibit Hall/Savannah International Trade and Convention Center
Date: Monday, August 1st, 2016
Time: 5:30 PM This poster will be presented at 6:15 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PPY006
Abstract ID:403
Candidate for Awards:None