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Abstract Detail


Thapa, Ramhari [1], Bayer, Randall [2], Mandel, Jennifer R [3].

Reconstruction of Phylogenetic Relationship in Antennaria (Asteraceae) using Data from Hundreds of Loci.

The phylogenetic relationships among amphimictic, sexually reproducing Antennaria (Asteraceae) species are poorly understood. Antennaria is a genus of dioecious, perennial herbs with a broad distribution in the Holarctic region except for the three species found in the Andes of South America. Previous morphology-based cladistic approaches and also molecular work utilizing data from nuclear ribosomal DNA internal transcribed spacers (ITS) did not produce a well-supported phylogenetic tree. We employed a novel target enrichment method using a set of custom capture probes designed to work in species across the entire Asteraceae family, coupled with next generation sequencing (NGS) to gather phylogenetic information from hundreds of nuclear loci. We sequenced thirty-four amphimictic Antennaria species including newly discovered species A. sawyeri and several outgroup taxa, and used bioinformatic and phylogenetic programs to produce a phylogenetic tree to understand the evolutionary history in the group. We also assembled partial chloroplast genomes for these species using the off-target reads to strengthen support for major relationships. Most areas of the tree are well-resolved with this approach, however some relationships are still unknown, and we are continuing to address these areas. The successful reconstruction of phylogeny across the genus would be a valuable tool to provide a framework for future evolutionary studies including species diversification, and the origination of polyploidy in Antennaria.

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1 - University of Memphis, Biological Sciences, 3700 Walker Avenue, Memphis,, TN - Tennessee, 38152, USA
2 - The University Of Memphis, Department Of Biological Sciences, 3700 Walker Avenue, 201 Ellington Hall, Memphis, TN, 38152, USA
3 - University of Memphis, Department of Biological Sciences, Memphis, TN, 38152, USA

Next generation sequencing

Presentation Type: Oral Paper
Session: 21, Cooley Awards I
Location: 201/Savannah International Trade and Convention Center
Date: Tuesday, August 2nd, 2016
Time: 10:30 AM
Number: 21010
Abstract ID:429
Candidate for Awards:George R. Cooley Award

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