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Abstract Detail

Genomics / Proteomics

Hall, Nathan [1], Zhang, Hui [2], McElroy, J. Scott [2], Goertzen, Leslie [1].

The Mitochondrial Genome of Eleusine indica.

Eleusine indica (Poaceae) is a widespread and problematic weed from eurasia commonly known as goosegrass. Broad ecological tolerance and prolific seeding ability have contributed to its spread throughout North America. E. indica is a close relative and likely progenitor of the allotetraploid cereal crop African Finger Millet (E. corocana), a highly nutritious and important food source in semiarid regions. The development of genomic resources for E. indica will provide insight into the basic biology of this hardy species and provide resources for the improvement of E. corocana. Here we describe the assembly and annotation of the goosegrass mitochondrial genome and compare it to other grass species. Accurate assembly of plant mitochondrial genomes from short reads can be limited by the repetitive and multipartite nature of this region in plants. Here we combined paired-end with mate-pair reads and explored various assembly algorithms and data visualization methods to better describe the nature of the mitochondrial genome in goosegrass. We identify 33 protein coding genes, 3 rRNAs and numerous tRNAs in approximately half a megabase of sequence and compare E. indica mt genome structure and content to other grasses in a phylogenetic context.

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1 - Auburn University, Biological Sciences, 101 Rouse Life Sciences Bldg., Auburn, AL, 36849, USA
2 - Auburn University, Department of Crop, Soil, and Environmental Sciences, Auburn, Al, 36849

Eleusine indica

Presentation Type: Oral Paper
Session: 19, Genomics & Proteomics I
Location: 105/Savannah International Trade and Convention Center
Date: Tuesday, August 2nd, 2016
Time: 11:45 AM
Number: 19015
Abstract ID:443
Candidate for Awards:Margaret Menzel Award

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