Abstract Detail

Pteridology

Ludwig, Thomas [1], Catron, Ashley [1], Link-Perez, Melanie [2].

Identifying Hybrid Origins of Species in the genus Adiantopsis Fée using Recombinant DNA and Cloning.

It is a common occurrence among ferns for hybridization to lead to allopolyploid species. One genus in which this likely has occurred is Adiantopsis Fée, a genus of 30-40 species in the Pteridaceae. Within Adiantopsis are several taxa, such as A. lindigii and A. pentagona, which published data suggests have hybrid origins, while other species are hypothesized to be contributing to the formation of hybrids. These hypotheses are based on morphological data, such as spore size compared to known diploids and intermediate phenotypes, and molecular data that place some species in phylogenetic positions that are suggestive of reticulate evolution. To investigate the possible role of hybridization and polyploidy in the origin of species in Adiantopsis, we are amplifying three nuclear genes followed by cloning. These techniques allow us to isolate various forms of genes, if various forms exist, and identify progenitor taxa. The target genes are gapCp, CRY, and SQD, which code for glyceraldehyde-3-phosphate dehydrogenase, cryptochrome, and sulfoquinovosyl diacylglycerol, respectively. Preliminary results for both gapCp and CRY support several hypotheses relating to Caribbean taxa and are revealing some surprises for several South American species, such as A. hickeyii (ined.). We currently are collecting data for SQD and will present highlights from all three datasets.

Related Links:
Link-Perez Lab

1 - Armstrong State University, 11935 Abercorn Street, Savannah, Georgia, 31419, United States
2 - Armstrong State University, Biology, 11935 Abercorn St., Savannah, GA, 31419, USA

Keywords:
fern
cloning
Hybrids
polyploidy
reticulate evolution.

Presentation Type: Poster
Session: P, Pteridological Section/AFS Posters
Location: Exhibit Hall/Savannah International Trade and Convention Center
Date: Monday, August 1st, 2016
Time: 5:30 PM This poster will be presented at 6:15 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PPD002
Abstract ID:468
Candidate for Awards:None