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Abstract Detail



Seed-free Plants at the Genomic Scale

McManus, Hilary A [1].

A comparison of chloroplast and mitochondrial genomes within a family of green algae.

The diversity of green algal lineages at the organellar genome level is recently garnering more attention, highlighting various trends in genome size, architecture, and gene content. A majority of these comparisons are across orders and classes, with relatively little focus on within-family comparisons. In an effort to increase our understanding of organellar genome evolution in the green algae, chloroplast and mitochondrial genome sequence data were collected from the freshwater family Hydrodictyaceae (Sphaeropleales, Chlorophyceae). The family comprises the morphologically diverse genera Pediastrum and Hydrodictyon, that form microscopic and macroscopic coenobia, respectively. In 2005, phylogenetic analyses of the family using nuclear 18S, ITS-2 and 28S rDNA data resulted in taxonomic revisions, however additional sampling and phylogenetic studies have been unable to resolve critical relationships necessary for further taxonomic revisions. Complete chloroplast and mitochondrial genomes of the hydrodictyaceaen taxa Stauridium tetras, Pseudopedistrum boryanum, Pediastrum duplex, Pediastrum angulosum, Lacunastrum gracillimum, and Hydrodictyon reticulatum, representing six phylogenetic groups, are characterized. The chloroplast genomes range in size from 143,193 bp to 225,641 bp, while the mitochondria range from 37,723 to 53,560 bp. The size variability of the organelles is primarily due to intergenic region expansion. Each chloroplast genome sampled contains an inverted repeat. A comparison of the genomes tests for phylogenetic patterns of gene rearrangements and architecture that may aid in resolving phylogenetic relationships and support necessary taxonomic revisions.


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1 - Le Moyne College, Biological Sciences, 1419 Salt Springs Rd., Syracuse, NY, 13214, United States

Keywords:
Chlorophyceae
Sphaeropleales
phylogenomics
chloroplast
Mitochondria
green algae.

Presentation Type: Colloquium Presentations
Session: C4, Seed Free Plants at the Genomic Scale
Location: Chatham Ballroom - C/Savannah International Trade and Convention Center
Date: Wednesday, August 3rd, 2016
Time: 1:45 PM
Number: C4002
Abstract ID:563
Candidate for Awards:None


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