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Abstract Detail


Xiang, Qiu-Yun (Jenny) [1], Dong, Yibo [1], Douglas, Norman [2], Ji, Xiang [3], Obata, Shihori [1], Pais, Andrew [1], Peet, Robert [4].

Phylogeography of Nyssa sylvatica complex (Nyssaceae, Cornales) – Integrative evidence from GBS data and gene sequencing.

Genotyping by sequencing (GBS) has been shown to be a cost-effective useful approach for phylogeographic and population genomic studies. Due to some potential limitations of the approach, especially potentially large amounts of missing data, one is often left wondering how comparable the results are with those from regular DNA sequencing. We conducted a GBS study of the Nyssa sylvatica complex from the eastern United States, which is sister to Nyssa sinensis in China, and compared the results with analyses of plastid matK and trnH-K and nuclear antR and LFY sequences. The species complex consists of three previously recognized species (N. sylvatica, N. biflora, an N. ursina) and four sometimes-recognized varieties within N. sylvatica that are difficult to distinguish due to complex morphological variation. We sampled across a wide range of the natural distribution from over 90 locations. The GBS data were generated using Illumina NextSeq and gene sequence data were generated with Sanger sequencing. Analyses of these data were performed to answer the following questions: (1) Is there evidence supporting three species and four varieties as defined by morphology? (2) What is the pattern of evolutionary divergence, and is it spatially or taxonomically structured? (3) Are the results from Sanger sequencing data congruent with results from GBS data? Our results from analyses of phylogeny, gene genealogy, population structure, and genetic variation pattern were largely in concert between GBS and gene sequence data in suggesting two genetic groups with evident gene flow and most genetic variation occurring within the genetic groups. Samples of N. ursina and N. bilfora are in general placed in the same group with a few exceptions, while samples N. sylvatica are spread in both groups, showing little correspondence between the genetic boundary and taxonomic identity. The current range, mating system, ecology, and high levels of phenotypic diversity in the species complex are consistent with these interpretation.

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1 - North Carolina State University, Department of Plant and Microbial Biology, Gardner Hall 2115, Raleigh, NC, 27695-7612, United States
2 - University of Florida, Department of Biology, PO Box 118525, Gainesville, FL, 32611
3 - North Carolina State University, Bioinformatics Research Center, Raleigh, North Carolina, 27695
4 - University of North Carolina, Biology, 413 Coker Hall, Chapel Hill, NC

Genotyping by sequencing (GBS)
taxonomically complex group.

Presentation Type: Oral Paper
Session: 16, Phylogenomics II
Location: 202/Savannah International Trade and Convention Center
Date: Tuesday, August 2nd, 2016
Time: 9:15 AM
Number: 16006
Abstract ID:574
Candidate for Awards:None


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