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Abstract Detail


Lindo, Julieta [1], Dong, Yibo [2], Obata, Shihori [3], Ji, Xiang [4], Xiang, Qiu-Yun (Jenny) [5].

Phylogenomic study of Blue/White-Fruited Dogwoods (Cornus, Cornaceae) using GBS data.

Cornus (Cornaceae) is a temperate and subtropical genus with ca. 58 species of small trees and shrubs that are commonly cultivated as ornamentals. Several phylogenetic studies based on molecular and morphological data have been conducted in the past to understand phylogenetic relationship within the genus. These studies revealed four major lineages different in morphology. These are the Big-Bracted dogwoods (BB), the Dwarf Dogwoods (DW); the Cornelian Cherries (CC), and the Blue- or White-fruited dogwoods (BW). Among them, the BW group is the largest, consisting of approximately 35 species with the diversity centered in eastern Asia and eastern North America, and a few species in Europe, western North America, and South America. Species relationships within the group have remained poorly resolved, hampering evolutionary studies (such as biogeography and character evolution) and classification that relies on a well-resolved and strongly supported phylogeny. A variety of new methods are available for generating genome-wide data for phylogenomic analyses. Genotype By Sequencing (GBS) was recently shown to be a cost-effective method for resolving species relationships in a genus. The objective of my study is, therefore, to generate Genotype By Sequencing (GBS) data for the BW species and explore their utility in resolving species relationships through phylogenomic analyses. A total of 110 accessions representing all BW species and outgroup taxa were included in the GBS library for 75 bp sequencing by Illumina NexSeq at GSL. After filtering the data, matrices containing different levels of missing data were generated using the program pyRAD for phylogenetic analyses. Phylogenetic analyses were conducted using RAxML implemented in CIPRES Gateway using GTRCAT model and 100 replicates of bootstrap. Results showed grouping of samples of the same species, and pattern of species relationships is similar the previous study based on ITS and matK data with a few exceptions. However better resolution and greater nodal support were obtained for analyses of the GBS data. Two eastern Asian-eastern North American disjunct lineages were identified. The phylogeny is used to examine the biogeographic history and character evolution.

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1 - Plant And Microbial Biology, North Carolina State University, Gardner Hall 2115, Raleigh, NC, 27695-7612, USA
2 - North Carolina State University, Plant and Microbial Biology, Raleigh, NORTH CAROLINA, 27695, United States
3 - North Carolina State University, Plant and Microbial Biology, Raleigh, North Carolina, 27695
4 - North Carolina State University, Bioinformatics Research Center, Raleigh, North Carolina, 27695
5 - North Carolina State University, Gardner Hall 2115, CAMPUS BOX 7612, RALEIGH, NC, 27695-7612, USA

Genotyping by sequencing (GBS).

Presentation Type: Oral Paper
Session: 16, Phylogenomics II
Location: 202/Savannah International Trade and Convention Center
Date: Tuesday, August 2nd, 2016
Time: 10:15 AM
Number: 16009
Abstract ID:620
Candidate for Awards:None

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