Abstract Detail

Conservation Biology

Irvin, Lani [1], Vaidya, Brajesh [2], Arun, Alok [3], Joshee, Nirmal [2].

DNA Barcoding Studies in the Medicinal Genus Scutellaria.

The genus Scutellaria, a member of the Lamiaceae family, has about 400 species spread all over the Northern hemisphere. There are 20 species in our germplasm collection representing all major growing areas in different continents. Scutellaria is a medicinal plant with high anti-oxidant and anti-tumor activity that has been observed in our ongoing studies. Many species are rare, threatened or endangered. DNA barcode studies were carried out to objectively identify plant samples specifically even from small, damaged, or industrially processed material. This property holds great promise in identifying adulteration in herbal supplement industry. For developing DNA barcodes, genomic DNA was extracted from leaf samples of 20 Scutellaria species using the cetyltrimethylammonium bromide (CTAB) method. The concentration and purity of the DNA was determined using NanoDrop and by amplifying the housekeeping gene, tubulin. After confirmation of DNA, four candidate DNA barcode genes, the ribosomal RNA maturase gene (matK; primer sets matK2.1-r and matK-3f1), the ribulose-1, 4-bisphospate carboxylase/oxygenase gene (rbcL; primer sets rbcL, rbcL2 and rbcL1-991), the chloroplast intergenic spacer (psbA-trnH) and the ribosomal intergenic spacer (ITS; primer set ITS2-3) were amplified to discriminate between the Scutellaria species via polymerase chain reaction (PCR). PCR amplification conditions were optimized for each gene-primer combination to amplify a single discreet band. The amplicons were verified by gel electrophoresis and were sent for sequencing. After the sequencing is completed and bioinformatics analyzed, the DNA sequences will be uploaded to the Barcode of Life Data System (BOLD) and Basic Local Alignment Search Tool (BLAST) database where the data will be analyzed.

1 - Fort Valley State University, Biotechnology , 1005 State University Drive, Fort Valley, Georgia, 31030, United States
2 - Fort Valley State University, 1005 State University Drive, Fort Valley, Georgia, 31030, United States
3 - Inter-American University of Puerto Rico, Barranquitas, 00957, Puerto Rico

Keywords:
Scutellaria
DNA barcoding
adulteration.

Presentation Type: Poster
Session: P, Conservation Biology Posters
Location: Exhibit Hall/Savannah International Trade and Convention Center
Date: Monday, August 1st, 2016
Time: 5:30 PM This poster will be presented at 6:15 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PCB010
Abstract ID:645
Candidate for Awards:None