| Abstract Detail
Genomics / Proteomics An, Hong [1], Qi, Xinshuai [2], Barker, Michael S [3], Pires, Joseph Chris [4]. The origin and diversity of Brassica napus. Brassica napus is a worldwide multiuse crop, especially as a very important edible oil resource. It is an allopolyploid species naturally hybridized from Brassica rapa (AA=20) and Brassica oleracea (CC=19) about 7,500 years ago. Characterization of their origin and population structure is essential for Brassica napus germplasm conservation and heterosis utilization. We used 2*250 bp RNA-seq to generate transcriptomic data of 162 Brassica rapa, 40 Brassica oleracea, 93 Brassica napus and some other close related Brassicaceae species. Using these data, about 10 million SNPs were identified and used for population structure and phylogeny analysis. Based on these results, we confirmed that Brassica rapa is definitely a parent of Brassica napus, but we found that Brassica oleracea may have less contribution than Brassica rapa or no contribution to Brassica napus. Additionally, the spring type oilseed Brassica napus can be clearly distinguished from the winter type ones, and the East Asian winter type is also significantly different from the European and American winter type. Besides, we also evaluated the expression level of different subpopulations and different duplicated genes in Brassica napus and classified their different expressed genes into different functional categories.
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1 - University of Missouri-Columbia, Biological Sciences, 311 Bond Life Science Center, Columbia, Missouri, 65201, United States
2 - University of Arizona, Ecology & Evolutionary Biology, Tucson, AZ, 85721, USA
3 - University Of Arizona, Department Of Ecology & Evolutionary Biology, P.O. Box 210088, Tucson, AZ, 85721, USA
4 - University Of Missouri, 371 Bond Life Sciences Center, 1201 Rollins Street, Columbia, MO, 65211-7310, USA
Keywords:
Brassica napus
genetic structure
RNA-seq
Allopolyploidy.
Presentation Type: Oral Paper
Session: 27, Genomics & Proteomics II
Location: 105/Savannah International Trade and Convention Center
Date: Tuesday, August 2nd, 2016
Time: 1:45 PM
Number: 27002
Abstract ID:694
Candidate for Awards:None |
