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Abstract Detail


Gostel, Morgan [1], Dikow, Rebecca [2], Freitas, Karen [3], Sancho, Gisela [4], Roque, Nádia [5], Funk, Vicki [6].

Exploring PCR-based target enrichment applications for a 'critically placed tribe' in Compositae: Rapid radiations in South American Moquiniastrum (Gochnatieae).

Moquiniastrum (Gochnatieae) is a genus comprising 21 species belonging to a "critically placed tribe" near the base of the Compositae. Distributed throughout much of South America, this genus is characterized by diverse morphologies, habits, and a slightly disjunct distribution in Argentina, Bolivia, Brazil, Paraguay, Peru, Uruguay, and Venezuela. Recent molecular phylogenetic studies that have sampled heavily from this genus suggest that current molecular markers are insufficient to accurately reconstruct the evolutionary history of this genus. Utilizing 15 transcriptomes available through the 1KP project, we have designed and screened a suite of new markers for PCR-based target enrichment and anticipate these new markers are sufficient to reconstruct recent radiations that have occurred in the South American genus Moquiniastrum and perhaps more broadly in the Compositae. We outline our marker design strategy and validation success, as well as implications for marker development in this diverse family of angiosperms.

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1 - Smithsonian Institution, Department of Botany, PO Box 37012, MRC 166, Washington, DC, 20013, United States
2 - Smithsonian Institution, Office of Research Information Services, Office of the CIO, PO Box 37012, MRC 166, Washington, DC, 20013, United States
3 - Universidade Federal de Rio Grande do Sul, Departamento de Botânica, Av. Bento Gonçalves, 9550, Campus do Vale, Bloco IV - Prédio 43433, Porto Alegre, RS, 1501-970, Brasil
4 - Museo de La Plata, División Plantas Vasculares, Paseo del Bosque s.n., La Plata, Buenos Aires, Buenos Aires, 1900, Argentina
5 - Universidade Federal da Bahia, Intituto de Biologia, Rua Barão de Jeremoabo, Bairro Ondina, Salvador, Bahia, 40170-115, Brasil
6 - Smithsonian Institution, Department of Botany, US National Herbarium, NMNH, MRC166, P.O. Box 37012, Washington DC, DC, 20013-7012, USA, 202-6330950

target enrichment
High-Throughput Sequencing

Presentation Type: Oral Paper
Session: 46, Asterids I part C & Asterids II
Location: 103/Savannah International Trade and Convention Center
Date: Wednesday, August 3rd, 2016
Time: 4:15 PM
Number: 46010
Abstract ID:784
Candidate for Awards:None

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