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Abstract Detail



Systematics

Gardner, Elliot [1], Johnson, Matthew [2], Pereira, Joan T. [3], Raguso, Robert [4], Skogen, Krissa [2], Wickett, Norm [2], Zerega, Nyree J.C. [1].

Phylogenomics of Artocarpus (Moraceae) from 333 nuclear genes: insights into pollination transitions.

Artocarpus (Moraceae) is a rare example of a clade exhibiting animal pollination derived from ancestral wind pollination. With a center of diversity in the lowland forests of Southeast Asia, the genus contains approximately 70 species of monecious trees, including notable underutilized crops such as breadfruit (A. altilis) and jackfruit (A. heterophyllus). Although ancestral Moraceae were wind pollinated, both wind and animal pollination are found in Artocarpus, including a unique mutualism mediated by parasitic fungi that provide a brood site and nutrition for dipteran pollinators. To investigate the transition from wind to animal pollination, we performed a phylogenomic reconstruction of Artocarpus based on target-enrichment sequencing (HybSeq) of 360 low-copy nuclear genes, using material ranging from freshly-collected plants to historical specimens up to 100 years old. The targets were developed from a de novo draft genome assembly (17x coverage) and annotation of A. camansi. Target sequences were assembled and processed with the HybPiper pipeline, and analyses included explorations of coding vs. non-coding regions and gene paralogy, and comparisons between supermatrix and species-tree approaches, resulting in a well-resolved phylogeny with over 90% taxon sampling. We combined the phylogeny with trait data including results of pollinator-exclusion experiments and floral volatile analysis to illuminate characters associated with wind and animal pollination.


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1 - Chicago Botanic Garden and Northwestern University, 2205 Tech Dr., Hogan 2-144, Evanston, IL, 60208, United States
2 - Chicago Botanic Garden, Plant Science, 1000 Lake Cook Road, Plant Science Center, Glencoe, IL, 60022, USA
3 - Sabah Forestry Department, Forest Research Centre, Jalan Sepilok, Sandakan, Sabah, 90175, Malaysia
4 - Cornell University, Dept. of Neurobiology and Behavior, Room W355, Seeley G Mudd Hall, 215 Tower Road, Ithaca, NY, 14853, USA

Keywords:
Moraceae
Artocarpus
Underutilized crops
phylogenomics
pollination
floral volatiles
hyb-seq
target enrichment.

Presentation Type: Oral Paper
Session: 31, Cooley Awards II
Location: 201/Savannah International Trade and Convention Center
Date: Tuesday, August 2nd, 2016
Time: 3:45 PM
Number: 31009
Abstract ID:791
Candidate for Awards:George R. Cooley Award


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