Abstract Detail

Phylogenomics

Warschefsky, Emily [1], von Wettberg, Eric [1].

A fruitful endeavor: Using RAD-seq to reveal the evolutionary relationships of the mango genus.

Crop wild relatives have recently garnered much attention for their potential as reservoirs of genetic diversity that can be used for crop breeding and improvement. In tree crops, such as mango (Mangifera indica L.), these wild relatives are of additional value as potential rootstocks onto which cultivars can be grafted. Because graft compatibility between two species depends on a complex suite of characteristics, including genetic similarity, rootstock selection can be informed by a systematic understanding of the crop and its wild relatives. The mango genus, Mangifera, is one of the largest in the family Anacardiaceae, with the latest revision including 69 species spanning from Eastern India to the Solomon Islands. In addition to the economically important M. indica (mango), some 26 other species are regionally cultivated for their edible fruits, and many others present potentially beneficial traits such as salinity tolerance and disease resistance. Previous work based on morphological evidence proposed the delimitation of Mangifera into two subgenera and six sections, but published gene trees have called into question this classification. Here, we present the first multilocus molecular phylogeny for Mangifera, which includes representatives from all subgenera and sections. Reduced representation genomic data were obtained by double digest restriction site associated DNA (ddRAD) sequencing. Reads were quality filtered, then processed with the software pyRAD to identify single nucleotide polymorphisms. Phylogenetic reconstruction was performed using both Bayesian and Maximum Likelihood frameworks. The monophyly of currently delimited subspecies and sections was tested using Shimodaira-Hasegawa (SH), Kishino-Hasegawa (KH), and approximately unbiased (AU) tests. These results greatly improve our understanding of the evolutionary history of Mangifera, and indicate a clade of species that is most likely to be of use to mango growers. In addition, this research lays the groundwork for further investigations into the morphological diversification and phylogeography of this globally and regionally important genus.

1 - Florida International University & , Fairchild Tropical Botanic Garden, 11200 SW 8th st, Miami, FL, 33199, United States

Keywords:
phylogenomics
Anacardiaceae
RAD-seq
perennial crop
crop wild relative.

Presentation Type: Oral Paper
Session: 38, Phylogenomics III
Location: 204/Savannah International Trade and Convention Center
Date: Wednesday, August 3rd, 2016
Time: 10:15 AM
Number: 38009
Abstract ID:796
Candidate for Awards:None