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Abstract Detail



Bryology and Lichenology

Greenwood, Joshua L [1].

Exposure to slowly applied desiccating conditions hardens Physcomitrella patens against subsequent rapidly applied desiccation stress.

The project I am presenting is centered upon the ability of Physcomitrella patens, to undergo physiological hardening in response to hydration stress eliciting a response capable of surviving a rapidly applied desiccated state. I show for the first time that physiological hardening occurs in P. patens and describe the period for which this hardening lasts. Organisms were exposed to a hardening treatment of two rates of slow drying to 50% RH (one at 4 days in length and another at 8). Following rehydration from this hardening samples were given one, four, eight, or twelve days of constant hydration (deacclimation) followed by a rapid drying event in which samples were dried to 50% RH in under an hour. A rapid dry event is lethal to unhardened members of this species and serves as a test to examine whether the previous exposure to a slow drying has resulted in hardening. The length of time spent hydrated before the rapid drying event allowed for determination of how long the hardening would be maintained in the absence of stimuli promoting tolerance of desiccation. Evidence gathered so far indicates that hardening can be obtained by a slow drying event and that protection lasts up to four days following the initial stimuli. Survival rates between hardened and unhardened samples are dramatically different with survival much higher in hardened tissues compared against unhardened tissues when both were subjected to a rapid drying event.


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1 - UNLV, School of Life Sciences, 4505 Maryland Parkway, Las Vegas, Nevada, 89154-4004, United States

Keywords:
Desiccation
Hardening
Abiotic Stress.

Presentation Type: Oral Paper
Session: 8, Bryology and Lichenology (ABLS) I
Location: 205/Savannah International Trade and Convention Center
Date: Monday, August 1st, 2016
Time: 11:15 AM
Number: 8009
Abstract ID:797
Candidate for Awards:None


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